Morphogenesis of Cymbidium atropurpureum In-Vitro

Main Article Content

Gayathery Subramaniam
Rosna Mat Taha

Abstract

Shoot tips excised from the orchid, $Cymbidium$ $atropurpureum$, produced protocorm-like bodies (PLB) on hormone-free Vacin and Went medium and hormone supplemented Vacin and Went (VW) medium. Explants showed the highest PLB formation (63.33%) on the medium supplemented with 5.0 mg/l of $\alpha$-naphthaleneacetic acid (NAA). The PLBs obtained were regenerated on hormone-free Murashige and Skoog (MS) medium. Besides PLB formation, callus formation from shoot tips was also observed in this study. Pseudobulb, rhizome and root explants produced callus on 1/2 strength MS mdium supplemented with 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l of thiadiazuron. The callus was maintained by sub-culturing in the same medium. Further experiments to determine if the callus obtained is organogenic or embryogenic is in progress.

Downloads

Download data is not yet available.

Article Details

How to Cite
Subramaniam, G., & Mat Taha, R. (2003). Morphogenesis of Cymbidium atropurpureum In-Vitro. Malaysian Journal of Science, 22(1), 1–5. Retrieved from https://mjes.um.edu.my/index.php/MJS/article/view/8791
Section
Original Articles